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Publikálva:

 

Lőrincz, A. – Neményi, M. (2002): Assessment of the effectiveness of ultrasonic cell disruption by acoustic phenomena as a function of the suspension concentration. 32’nd Annual Ultrasonic Industry Association Symposium. October 21 – 23. 2002, The Helmsley Hotel, New York, NY. Medical Session.

 

Assessment of the effectiveness of ultrasonic cell disruption by acoustic phenomena as a function of the suspension concentration

 

In our experiments, under the conditions of an output of 9 W/cm2 and a frequency of 1.117 MHz, we determined the concentration, expressed in [g/l], of lyophilized Saccharomyces cerevisiae baker’s yeast needed for stopping cavitation in the sound field. Then by using multiples of the aforementioned concentration, we monitored the acoustic phenomena occurred in the sound field and, simultaneously, we examined the survival dynamics of the cells by vitality dyeing. Examined acoustic phenomena were the following: acoustic streaming, standing wave, and cavitation. Physical parameters of the sound field had essential effect on the phenomena formed in the sound field and on the threshold levels of their formation. The phenomena affected the composition of the material in the sound field, so a phenomena – effect chain reaction took place during the radiation. This paper helps in understanding the cell destruction effect caused by the different phenomena and the role of physical conditions of the sound field in the formation of the acoustic phenomena.

 

Key Words: Acoustic phenomenon, cavitation, standing wave, acoustic streaming, yeast, R, D values, sound field