Lőrincz, A. (2004): Opportunities for Applying Digital and Analogous Machine Vision in Cell Analytical Methods Used for Analyzing Cell Disruption Effect of Ultrasound. XXII International Congress of the International Society for Analytical Cytology (ISAC), 22-27 May 2004 at Le Corum in Montpellier, France.
Opportunities for Applying Digital and Analogous Machine Vision in Cell Analytical Methods Used for Analyzing Cell Disruption Effect of Ultrasound
UltraSonoTech Laboratory; H-9200 Mosonmagyaróvár, Magyar u. 17. Hungary
During the examinations in vitro Saccharomyces cerevisiae yeast samples were irradiated with ultrasound having an output power of 9 W cm-2 and a frequency of 1.117 MHz. The vitality of the cells during the ultrasound treatment was examined by vital staining. Color and brightness of the cells was changed immediately when they were destroyed. The treated yeast sample was circulated in a flow-injection system between the ultrasound irradiation field and the optically detector. Light microscope equipped with a CCD camera was used as an optical detector. Slide of the light microscope was replaced by an optical flow-injection cell and the treated sample was circulated through this. From the image of CCD camera the survival dynamics and the decimation periods of the examined species were determined by using analogous and digital machine vision methods. For analogous machine vision, a pseudo-color coding system was built that was capable of evaluating different degree of the grey scale of CCD image. For digital imaging software was used. During the active ultrasound irradiation, a series of acoustic phenomena occurred in the suspended samples and each phenomenon has different intensity of cell damaging effect. This provided a good opportunity for the manual validation of the survival results determined by machine vision methods. By comparing the different examined methods, conclusions regarding their practicability and limitations in determining the number of living cells were drawn. By means of the applied machine vision systems, the quantity and quality of the individual cell types and cell organelles and for example, the environmental toxicity can be determined in real time.
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