Publikálva:
Lőrincz, A. (2004): Opportunities for Applying Digital
and Analogous Machine Vision in Cell Analytical
Methods Used for Analyzing Cell
Disruption Effect of Ultrasound. XXII International Congress of the International
Society for Analytical Cytology (ISAC), 22-27
May 2004 at Le Corum in Montpellier,
France.
Opportunities for Applying Digital and Analogous
Machine Vision in Cell Analytical Methods Used for Analyzing Cell Disruption
Effect of Ultrasound
A. LŐRINCZ* UltraSonoTech Laboratory; H-9200 Mosonmagyaróvár, Magyar u. 17. Hungary During the examinations in vitro Saccharomyces
cerevisiae yeast samples were irradiated with
ultrasound having an output power of 9 W cm-2 and a frequency of
1.117 MHz. The vitality of the cells during the ultrasound treatment was
examined by vital staining. Color and brightness of the cells was changed
immediately when they were destroyed. The treated yeast sample was circulated
in a flow-injection system between the ultrasound irradiation field and the optically
detector. Light microscope equipped with a CCD camera was used as an optical
detector. Slide of the light microscope was replaced by an optical
flow-injection cell and the treated sample was circulated through this. From
the image of CCD camera the survival dynamics and the decimation periods of the
examined species were determined by using analogous and digital machine vision
methods. For analogous machine vision, a pseudo-color coding system was built
that was capable of evaluating different degree of the grey
scale of CCD image. For digital imaging software was used. During the active
ultrasound irradiation, a series of acoustic phenomena occurred in the
suspended samples and each phenomenon has different intensity of cell damaging
effect. This provided a good opportunity for the manual validation of the
survival results determined by machine vision methods. By comparing the
different examined methods, conclusions regarding their practicability and
limitations in determining the number of living cells were drawn. By means of
the applied machine vision systems, the quantity and quality of the individual
cell types and cell organelles and for example, the environmental toxicity can
be determined in real time. |
